<em>In Vitro</em> Growth of Mouse Preantral Follicles Under Simulated Microgravity
نویسندگان
چکیده
منابع مشابه
In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
14 day-old mouse ovarian tissue and preantral follicles isolated from same-aged mice were incubated in a simulated microgravity culture system. We quantitatively assessed follicle survival, measured follicle and oocyte diameters, and examined ultrastructure of the oocytes produced from the system. We observed decreased follicle survival, downregulation of expressions of proliferating cell nucle...
متن کاملSimulated Microgravity Using a Rotary Culture System Compromises the In Vitro Development of Mouse Preantral Follicles
BACKGROUND Growing cells in simulated weightlessness condition might be a highly promising new technique to maintain or generate tissue constructs in a scaffold-free manner. There is limited evidence that microgravity condition may affect development of ovarian follicles. The objective of the present study was to investigate the effects of simulated microgravity on the in vitro development of m...
متن کاملP-107: Vitrification and In Vitro Culture of Mouse Preantral Follicles in Presence of Growth Factors
Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...
متن کاملMeiotic progression of isolated mouse spermatocytes under simulated microgravity.
Progression through the prophase of the first meiotic division can be obtained in culture by treatment of mouse spermatocytes with the serine/threonine phosphatase inhibitor okadaic acid. Chromosome condensation during this G2/M transition involves the activation of the MAPK pathway, which causes the activation of Nek2 and the phosphorylation of the chromatin architectural protein Hmga2. In an ...
متن کاملVitrification and Subsequent In Vitro Maturation of Mouse Preantral Follicles in Presence of Growth Factors
OBJECTIVE Cryopreservation of ovarian tissue or follicles has been proposed as an alternative method for fertility preservation. Although successful vitrification of follicles has been reported in several mammalian species, the survival rate is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and epidermal growth factor (EGF) on in vitro prea...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2017
ISSN: 1940-087X
DOI: 10.3791/55641